The investigation of the stability of DNA-<italic>b</italic>-PPO vesicles formed through frame guided assembly

Bo Bian; Yi-Yang Zhang; Yuan-Chen Dong; Fen Wu; Chao Wang; Shuo Wang; Yun Xu; Dong-Sheng Liu

doi:10.1007/s11426-018-9309-5

SCIENCE CHINA Chemistry

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SCIENCE CHINA Chemistry, Volume 61, Issue 12: 1568-1571(2018) https://doi.org/10.1007/s11426-018-9309-5

The investigation of the stability of DNA-b-PPO vesicles formed through frame guided assembly

Bo Bian¹, Yi-Yang Zhang¹, Yuan-Chen Dong^1,*, Fen Wu², Chao Wang¹, Shuo Wang¹, Yun Xu³, Dong-Sheng Liu^1,*

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ReceivedMay 10, 2018
AcceptedJun 8, 2018
PublishedJul 13, 2018

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Abstract

Frame-guided assembly is a novel assembly method which can control the shape and size of vesicles accurately. It has been reported that the DNA-b-PPO vesicles could be stable over two weeks. In this communication we have introduced restriction sites of EcoR1 in the interior DNA scaffold to the formed vesicles in order to further study their stability. Dynamic light scattering (DLS), transmission electron microscope (TEM) and fluorescence spectrum were used to characterize the digestion process. The final results revealed that the vesicles were still stable even after two weeks cultivated with EcoR1, which suggests potential in future biological applications.

Funded by

the National Basic Research Program of China(2013CB932803)

the National Natural Science Foundation of China(21534007)

and the Beijing Municipal Science & Technology Commission.

Acknowledgment

This work was supported by the National Basic Research Program of China (2013CB932803), the National Natural Science Foundation of China (21534007), and the Beijing Municipal Science & Technology Commission.

Interest statement

The authors declare that they have no conflict of interest.

Supplement

The supporting information is available online at http://chem.scichina.com and http://link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Figure 1
(a) DLS characterization of frame-guided assembly process. (b) The gel results of the hybridization and cleavage of the DNA-b-PPO. Lane 1, the ssDNA containing GAATTC. Lane 2, the complementary DNA sequence modified by PPO. Lane 3, the hybridization results of DNA-b-PPO and its complementary DNA before nuclease cultivation. Lane 4, the hybridized structure after nuclease cultivation for 12?h. (c) The TEM results of DNA vesicles upon formation and after cultivation with EcoR1 for 12?h. Scale bar=200 nm; Scale bar(inserted images)=100 nm.
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Scheme 1
The formation of vesicles and its cultivation with EcoR1. (a) The restriction sites were introduced in the DNA-b-PPO; (b) the restriction sites were introduced only in the shorter DNA attached on the AuNPs. The green star represented FAM fluorescent molecule and the yellow dsDNA meant that it contained restriction sites (color online).
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Figure 2
The DLS data of the AuNP-based vesicles (a) upon formation, (b) after 12?h and (c) after two weeks (color online).
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Figure 3
Fluorescence spectrum of DNA vesicles and DNA-AuNP hybrids (a1, b1) before cultivation and (a2, b2) their supernatant after cultivation for 12?h (color online).

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