Acknowledgment

This work was supported by the National Natural Science Foundation of China (31271681, 3140101312), the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences, and Jiangsu Agriculture Science and Technology Innovation Fund (CX(13)5075).

Open access

This article is distributed under the terms of the Creative Commons Attribution License, which permits any use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Interest statement

The author(s) declare that they have no conflict of interest.

Supplement

Figure S1 Mutation types at the eight target sites in the T₀ generation.

Table S1 Mutations detected in putative CRISPR/Cas9 off-target sites

Table S2 Primers used in this study.

The supporting information is available online at life.scichina.com and www.springerlink.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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